Article
Multiple Ras functions can contribute to mammalian cell transformation.
Cell - 24 Feb 1995
White M A, Nicolette C, Minden A, Polverino A, Van Aelst L, Karin M, Wigler M H
Abstract excerpt
We have developed a generalized approach, using two hybrid interactions, to isolate Ha-Ras effector loop mutations that separate the ability of Ha-Ras to interact with different downstream effectors. These mutations attenuate or eliminate Ha-ras(G12V) transformation of mammalian cells, but retain...
Topics
- Animals
- Cell Transformation, Neoplastic
- Cells, Cultured
- Chloramphenicol O-Acetyltransferase
- Gene Library
- Genes, ras
- Genetic Complementation Test
- Humans
- Mice
- Mutation
- Protein Kinases
- Protein Serine-Threonine Kinases
- Proto-Oncogene Proteins
- Proto-Oncogene Proteins c-raf
- Rats
- Recombinant Fusion Proteins
- Saccharomyces cerevisiae
- Schizosaccharomyces