Article
A novel allele of Saccharomyces cerevisiae RFA1 that is deficient in recombination and repair and suppressible by RAD52.
Molecular and cellular biology - 1 Mar 1995
Firmenich A A, Elias-Arnanz M, Berg P
Abstract excerpt
To understand the mechanisms involved in homologous recombination, we have performed a search for Saccharomyces cerevisiae mutants unable to carry out plasmid-to-chromosome gene conversion. For this purpose, we have developed a colony color assay in which recombination is induced by the controlle...
Topics
- Alleles
- Base Sequence
- Cloning, Molecular
- Crosses, Genetic
- DNA Repair
- DNA-Binding Proteins
- Deoxyribonucleases, Type II Site-Specific
- Fungal Proteins
- Gene Conversion
- Genes, Fungal
- Genotype
- Macromolecular Substances
- Molecular Sequence Data
- Mutagenesis
- Oligodeoxyribonucleotides
- Plasmids
- Rad52 DNA Repair and Recombination Protein
- Recombinant Proteins