Article
Involvement of glutamic acid 23 in the catalytic mechanism of T4 endonuclease V.
The Journal of biological chemistry - 10 Feb 1995
Manuel R C, Latham K A, Dodson M L, Lloyd R S
Abstract excerpt
Bacteriophage T4 endonuclease V has both pyrimidine dimer-specific DNA glycosylase and abasic (AP) lyase activities, which are sequential yet biochemically separable functions. Previous studies using chemical modification and site-directed mutagenesis techniques have shown that the catalytic acti...
Topics
- Amino Acid Sequence
- Bacteriophage T4
- Base Sequence
- Binding Sites
- Catalysis
- Crystallography, X-Ray
- DNA, Recombinant
- Deoxyribonuclease (Pyrimidine Dimer)
- Endodeoxyribonucleases
- Escherichia coli
- Glutamic Acid
- Molecular Sequence Data
- Molecular Weight
- Mutation
- Plasmids
- Protein Binding
- Pyrimidine Dimers
- Thymine