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Article

Potential use of a Yersinia ruckeri O1 auxotrophic aroA mutant as a live attenuated vaccine.

Journal of fish diseases - 1 Jul 2005

Temprano A, Riaño J, Yugueros J, González P, de Castro L, Villena A, Luengo J M, Naharro G

Abstract excerpt

The aroA gene of Yersinia ruckeri, which encodes 5-enolpyruvylshikimate 3-phosphate synthase, was insertionally inactivated with a DNA fragment containing a kanamycin resistance determinant and reintroduced by allelic exchange into the chromosome of Y. ruckeri 21102 O1 by means of the suicide vector pIVET8. The Y. ruckeri aroA::Kan(r) mutant was highly attenuated when inoculated intraperitoneally into rainbow...

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Identifiers

PMID: 16083447
DOI: 10.1111/j.1365-2761.2005.00646.x

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