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Article

Ligand binding to wild-type and E-B13Q mutant insulins: a three-state allosteric model system showing half-site reactivity.

Journal of molecular biology - 27 Jan 1995

Bloom C R, Choi W E, Brzovic P S, Ha J J, Huang S T, Kaarsholm N C, Dunn M F

Abstract excerpt

By using ultra-violet and visible absorbance in conjunction with high field 1H-nuclear magnetic resonance spectroscopy, the insulin hexamer has been shown to undergo two allosteric transitions in solution involving three allosteric states (T6<-->T3 R3<-->R6). A simple mathematical model consisting of four variables has been derived that quantitatively describes the complex homotropic and heterotropic interactions...

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Source

Identifiers

PMID: 7837266
DOI: 10.1006/jmbi.1994.0027

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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