Ligand binding to wild-type and E-B13Q mutant insulins: a three-state allosteric model system showing half-site reactivity.

doi:10.1006/jmbi.1994.0027

Back to search

Article

Ligand binding to wild-type and E-B13Q mutant insulins: a three-state allosteric model system showing half-site reactivity.

Journal of molecular biology - 27 Jan 1995

Bloom C R, Choi W E, Brzovic P S, Ha J J, Huang S T, Kaarsholm N C, Dunn M F

Abstract excerpt

By using ultra-violet and visible absorbance in conjunction with high field 1H-nuclear magnetic resonance spectroscopy, the insulin hexamer has been shown to undergo two allosteric transitions in solution involving three allosteric states (T6<-->T3 R3<-->R6). A simple mathematical model consistin...

Topics

Allosteric Site
Glutamic Acid
Glutamine
Insulin
Ligands
Magnetic Resonance Spectroscopy
Models, Chemical
Mutation
Spectrophotometry, Ultraviolet

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.