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Article

Role of B13 Glu in insulin assembly. The hexamer structure of recombinant mutant (B13 Glu-->Gln) insulin.

Journal of molecular biology - 20 Dec 1992

Bentley G A, Brange J, Derewenda Z, Dodson E J, Dodson G G, Markussen J, Wilkinson A J, Wollmer A, Xiao B

Abstract excerpt

The assembly of the insulin hexamer brings the six B13 glutamate side-chains at the centre into close proximity. Their mutual repulsion is unfavourable and zinc co-ordination to B10 histidine is necessary to stabilize the well known zinc-containing hexamers. Since B13 is always a carboxylic acid in all known sequences of hexamer forming insulins, it is likely to be important in the hormone's biology. The mutation...

Topics

Animals
Crystallization
Glutamates
Glutamic Acid
Glutamine
Humans
Insulin
Macromolecular Substances
Models, Molecular
Mutagenesis, Site-Directed
Mutation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Protein Conformation

Recombinant Proteins

Swine

X-Ray Diffraction

Zinc

Source

Identifiers

PMID: 1361949
DOI: 10.1016/0022-2836(92)90323-c

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1361949-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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