Article
High-resolution structure of an engineered biologically potent insulin monomer, B16 Tyr-->His, as determined by nuclear magnetic resonance spectroscopy.
Biochemistry - 5 Jul 1994
Ludvigsen S, Roy M, Thøgersen H, Kaarsholm N C
Abstract excerpt
Site-directed mutagenesis is used in conjunction with 1H nuclear magnetic resonance (NMR) and circular dichroism (CD) spectroscopy in order to find an insulin species amenable for structure determination in aqueous solution by NMR spectroscopy. A successful candidate in this respect, i.e., B16 Ty...
Topics
- Amino Acid Sequence
- Circular Dichroism
- Computer Simulation
- Humans
- Hydrogen-Ion Concentration
- Insulin
- Magnetic Resonance Spectroscopy
- Mathematical Computing
- Molecular Sequence Data
- Mutation
- Protein Conformation
- Protein Engineering
- Protein Folding