Article
A sensitive method for detection of mutations--a PCR-based RNase protection assay.
DNA and cell biology - 1 Jan 1995
Murthy K K, Shen S H, Banville D
Abstract excerpt
Several techniques are currently available for detecting point mutations in DNA. The most widely used methods either use hazardous chemicals (chemical mismatch cleavage) or can detect mutations only in short (200- to 500-bp) fragments (single-stranded conformational polymorphism and denaturing gradient gel electrophoresis). In an effort to develop a sensitive and reliable method for the detection of mutations in...
Topics
- Animals
- DNA Mutational Analysis
- Genetic Carrier Screening
- Genetic Techniques
- Mice
- Mutation
- Nucleic Acid Heteroduplexes
- Nucleic Acid Hybridization
- Polymerase Chain Reaction
- Protein Tyrosine Phosphatases
- RNA Probes