Article
Genotyping single point mutations in rd1 and rd8 mice using melting curve analysis of qPCR fragments.
Scientific reports - 28 Aug 2024
Schwämmle Melanie E, Bucher Felicitas, Schlunck Günther, Martin Gottfried
Abstract excerpt
PCR is tolerant to single nucleotide mismatches. Therefore, genotyping of point mutations by PCR requires special conditions for the amplification of allele-specific PCR fragments. MS-PCR (mutagenically separated PCR) is an improved version of ARMS (amplification refractory mutation system) in which additional nucleotide mismatches near the mutation site are used to separate the wt fragments from the mutant...
Topics
- Animals
- Mice
- Point Mutation
- Genotyping Techniques
- Genotype
- Alleles
- Real-Time Polymerase Chain Reaction
- Transition Temperature
- Polymorphism, Single Nucleotide
- DNA Primers
- Retinal Degeneration
- Polymerase Chain Reaction
- Nucleic Acid Denaturation