The "megaprimer" method of site-directed mutagenesis.

Abstract excerpt

We describe a simple and efficient method of mutagenesis which we term the "megaprimer" method. The method utilizes three oligonucleotide primers to perform two rounds of polymerase chain reaction. In the method, the product of the first polymerase chain reaction is used as one of the polymerase chain reaction primers (a "megaprimer") for the second polymerase chain reaction. When a phage promoter and a...

Topics

• Base Sequence
• DNA
• Factor IX
• Molecular Sequence Data
• Mutation
• Polymerase Chain Reaction
• Protein Biosynthesis