Article
A general and rapid mutagenesis method using polymerase chain reaction.
Gene - 2 Jul 1990
Herlitze S, Koenen M
Abstract excerpt
The construction of deletions, insertions and point mutations in DNA sequences is a powerful approach to analysing the function and structure of genes and their products. Here, we present a fast and efficient method using the polymerase chain reaction to introduce mutations into cDNAs coding for the alpha-, gamma- and epsilon-subunit of the rat muscle acetylcholine receptor. Two flanking primers and one mutant...
Topics
- Animals
- Base Sequence
- Chromosome Deletion
- Cloning, Molecular
- DNA
- DNA Transposable Elements
- Genes
- Muscles
- Mutation
- Nucleic Acid Amplification Techniques
- Polymerase Chain Reaction
- Rats