Article
Inactivation of epoxide hydrolase by catalysis-induced formation of isoaspartate.
FEBS letters - 14 May 2008
van Loo Bert, Permentier Hjalmar P, Kingma Jaap, Baldascini Helen, Janssen Dick B
Abstract excerpt
Epoxide hydrolases catalyze hydrolytic epoxide ring-opening, most often via formation of a covalent hydroxyalkyl-enzyme intermediate. A mutant of Agrobacterium radiobacter epoxide hydrolase, in which the phenylalanine residue that flanks the invariant catalytic aspartate nucleophile is replaced by a threonine, exhibited inactivation during conversion when the (R)-enantiomer of para-nitrostyrene epoxide was used...
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