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Article

Crystal structures of penicillin acylase enzyme-substrate complexes: structural insights into the catalytic mechanism.

Journal of molecular biology - 12 Oct 2001

McVey C E, Walsh M A, Dodson G G, Wilson K S, Brannigan J A

Abstract excerpt

The crystal structure of penicillin G acylase from Escherichia coli has been determined to a resolution of 1.3 A from a crystal form grown in the presence of ethylene glycol. To study aspects of the substrate specificity and catalytic mechanism of this key biotechnological enzyme, mutants were made to generate inactive protein useful for producing enzyme-substrate complexes. Owing to the intimate association of...

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Identifiers

PMID: 11601852
DOI: 10.1006/jmbi.2001.5043

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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