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Article

Complementation of a Streptomyces lividans Leu- mutant by the Actinoplanes teichomyceticus leuC gene.

Gene - 26 May 1995

Castelli P, Donadio S, Marinelli F, Borghi A, Sosio M

Abstract excerpt

A leucine auxotroph of Streptomyces lividans (Sl), designated PC196, was unable to convert alpha-isopropylmalate into the beta-isomer. A DNA fragment from Actinoplanes teichomyceticus (At) cloned into the Streptomyces vector pIJ702 complemented PC196. Sequence analysis of the 3.0-kb insert revealed one complete ORF with high similarity to other leuC genes encoding the large subunit of isopropylmalate isomerase...

Topics

Actinomycetaceae
Bacterial Proteins
Cloning, Molecular
Genes, Bacterial
Genetic Complementation Test
Hydro-Lyases
Isomerases
Molecular Sequence Data
Mutation
Streptomyces

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Identifiers

PMID: 7789819
DOI: 10.1016/0378-1119(95)00142-s

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7789819-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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