Cloning and characterization of a gene encoding a secreted tripeptidyl aminopeptidase from Streptomyces lividans 66.

doi:10.1128/aem.61.8.3145-3150.1995

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Article

Cloning and characterization of a gene encoding a secreted tripeptidyl aminopeptidase from Streptomyces lividans 66.

Applied and environmental microbiology - 1 Aug 1995

Butler M J, Binnie C, DiZonno M A, Krygsman P, Soltes G A, Soostmeyer G, Walczyk E, Malek L T

Abstract excerpt

The gene encoding a tripeptidyl aminopeptidase (Tap) from Streptomyces lividans was cloned by using a simple agar plate activity assay. Overexpression of the cloned gene results in the production of a secreted protein which has an apparent subunit molecular weight of 55,000 and is responsible for the major amino-terminal degradative activity in culture broths of S. lividans strains. A DNA sequence analysis...

Topics

Amino Acid Sequence
Aminopeptidases
Base Sequence
Cloning, Molecular
DNA Primers
DNA, Bacterial
Dipeptidyl-Peptidases and Tripeptidyl-Peptidases
Endopeptidases
Genes, Bacterial
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutation

Plasmids

Protein Sorting Signals

Restriction Mapping

Species Specificity

Streptomyces

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Identifiers

PMID: 7487044
DOI: 10.1128/aem.61.8.3145-3150.1995
PMCID: PMC167588

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7487044-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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