Detection and analysis of hepatitis C virus by a combined RT-PCR method: variation in the 5' non-coding region of the viral genome.

doi:10.1099/00222615-42-5-367

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Article

Detection and analysis of hepatitis C virus by a combined RT-PCR method: variation in the 5' non-coding region of the viral genome.

Journal of medical microbiology - 1 May 1995

Karachristos A, Linardopoulos S, Ergazaki M, Spandidos D A

Abstract excerpt

A combined reverse transcription-polymerase chain reaction (RT-PCR) method was employed for the detection of hepatitis C virus (HCV) RNA in serum from patients with chronic active hepatitis, with primers corresponding to the 5' non-coding region. The diagnosis was based on serological and biochemical methods and on liver biopsy. HCV-RNA was detected in 27 (90%) of 30 sera examined. The nucleotide sequence of...

Topics

Base Sequence
Consensus Sequence
DNA Primers
DNA, Viral
Genetic Variation
Genome, Viral
Hepacivirus
Hepatitis C
Hepatitis, Chronic
Humans
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Oligonucleotides

Oligonucleotides, Antisense

Open Reading Frames

Polymerase Chain Reaction

RNA, Viral

Sequence Alignment

Transcription, Genetic

Source

Open in PubMed

Identifiers

PMID: 7752217
DOI: 10.1099/00222615-42-5-367

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7752217-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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