Article
A novel nested reverse-transcriptase polymerase chain reaction method for rapid hepatitis C virus detection and genotyping.
Indian journal of medical microbiology - 1 Jan 2000
Saha K, Firdaus R, Biswas A, Mukherjee A, Sadhukhan P C
Abstract excerpt
PURPOSE: Rapid and specific detection of viral nucleic acid is increasingly important in the diagnosis of infectious diseases. The objective was to develop a rapid, efficient process of nucleic acid based detection of hepatitis C virus (HCV) infection for its diagnosis and treatment follow-up. MATERIALS AND METHODS: A two-step nested reverse-transcriptase polymerase chain reaction (RT-PCR) has been standardised...
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