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A novel, sensitive, and specific RT‐PCR technique for quantitation of hepatitis C virus replication

19 Dec 2006

Abstract excerpt

The detection of negative-strand hepatitis C virus (HCV) RNA is a hallmark of replication. A highly sensitive and specific method is required to quantify the very low level of replication inherent to in vitro infection systems. Based on reverse transcription with a tagged primer in the 5' non-coding region of the HCV genome, followed by a nested PCR with a second round of real-time PCR, a novel method is...

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PMID: 17177304
DOI: 10.1002/jmv.20773

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