Article
Production of isoamyl acetate in ackA-pta and/or ldh mutants of Escherichia coli with overexpression of yeast ATF2.
Applied microbiology and biotechnology - 1 Feb 2004
Vadali R V, Horton C E, Rudolph F B, Bennett G N, San K-Y
Abstract excerpt
The gene coding for alcohol acetyltransferase ( ATF2), which catalyzes the esterification of isoamyl alcohol and acetyl coenzyme A (acetyl-CoA), was cloned from Saccharomyces cerevisiae and expressed in Escherichia coli. This genetically engineered strain of E. coli produced the ester isoamyl acetate when isoamyl alcohol was added externally to the cell culture medium. Various competing pathways at the acetyl-CoA...
Topics
- Acetate Kinase
- Acetyltransferases
- Biotransformation
- Cloning, Molecular
- Escherichia coli
- Gene Deletion
- Gene Expression
- Genes, Bacterial
- Genes, Fungal
- L-Lactate Dehydrogenase
- Mutation
- Pentanols
- Phosphate Acetyltransferase
- Recombinant Proteins