Article
Development of a low-cost real-time reverse transcriptase-polymerase chain reaction technique for the detection and quantification of hepatitis C viral load.
Clinical chemistry and laboratory medicine - 1 Jun 2010
Shahzamani Kiana, Merat Shahin, Rezvan Houri, Mirab-Samiee Siamak, Khademi Hooman, Malekzadeh Reza, Sabahi Farzaneh
Abstract excerpt
BACKGROUND: It is necessary to develop a highly specific and sensitive assay to quantify the exact amount of hepatitis C virus (HCV) RNA in blood of patients with hepatitis C. For this reason, a real-time reverse transcriptase-polymerase chain reaction (RT-PCR) assay for quantification of HCV RNA in human plasma was developed. METHODS: A pair of primers as well as hybridization probes were selected. A real-time...
Topics
- Genotype
- Hepacivirus
- Hepatitis C
- Humans
- RNA, Viral
- Reproducibility of Results
- Reverse Transcriptase Polymerase Chain Reaction
- Sensitivity and Specificity
- Viral Load