Article
A C-terminal domain, which prevents secretion of the neuroendocrine protein 7B2 in Saccharomyces cerevisiae, inhibits Kex2 yet is processed by the Yap3 protease.
FEBS letters - 1 May 1995
Chaudhuri B, Stephan C
Abstract excerpt
Recent reports reveal that the C-terminal half of the neuroendocrine polypeptide 7B2 selectively inhibits and binds PC2, a mammalian prohormone converting enzyme that is homologous to the yeast pro-alpha-factor processing protease Kex2. During attempted secretion of the 185 amino-acid human 7B2 in Saccharomyces cerevisiae, we observe that the protein is mostly retained inside the cell. However a mutant...
Topics
- Aspartic Acid Endopeptidases
- Base Sequence
- DNA Mutational Analysis
- Endopeptidases
- Humans
- Molecular Sequence Data
- Mutation
- Nerve Tissue Proteins
- Neuroendocrine Secretory Protein 7B2
- Peptide Fragments