Article
Mutants of Escherichia coli integration host factor: DNA-binding and recombination properties.
Biochimie - 1 Jan 1994
Hales L M, Gumport R I, Gardner J F
Abstract excerpt
Integration host factor (IHF) is a protein encoded by Escherichia coli, which was first discovered as a requirement for bacteriophage lambda site-specific recombination. In this study, we characterized mutants of IHF for their ability to bind to various IHF binding sites in vivo and to promote recombination of lambda in vitro. DNA-binding in vivo was monitored using the challenge-phage system. If IHF binds to its...
Topics
- Bacterial Proteins
- Base Sequence
- Binding Sites
- DNA-Binding Proteins
- Escherichia coli
- Molecular Sequence Data
- Mutation
- Recombination, Genetic