Article
The isolation and characterization of mutants of the integration host factor (IHF) of Escherichia coli with altered, expanded DNA-binding specificities.
The EMBO journal - 1 Jan 1992
Lee E C, Hales L M, Gumport R I, Gardner J F
Abstract excerpt
The integration host factor (IHF) of Escherichia coli is a small, basic protein that is required for lambda site-specific recombination and a variety of cellular processes. It is composed of two subunits, alpha and beta, that are encoded by the himA and hip (himD) genes, respectively. IHF is a sequence-specific DNA-binding protein and bends the DNA when it binds. We have used the bacteriophage P22-based challenge...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Bacteriophage lambda
- Base Sequence
- Binding Sites
- DNA-Binding Proteins
- Escherichia coli
- Integration Host Factors
- Models, Molecular
- Molecular Sequence Data