Comparison of protein binding to DNA in vivo and in vitro: defining an effective intracellular target.

doi:10.1002/j.1460-2075.1995.tb00319.x

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Article

Comparison of protein binding to DNA in vivo and in vitro: defining an effective intracellular target.

The EMBO journal - 15 Dec 1995

Yang S W, Nash H A

Abstract excerpt

We have quantitatively evaluated the affinity of a set of target sites for the integration host factor (IHF) protein of Escherichia coli by their performance as competitors in an electrophoretic mobility shift assay. We also determined how well each of these sites is filled by IHF in vivo. The da...

Topics

Bacterial Proteins
Base Sequence
Binding Sites
DNA Footprinting
DNA, Bacterial
DNA-Binding Proteins
Escherichia coli
Genetic Variation
Integration Host Factors
Kinetics
Molecular Sequence Data
Protein Binding

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