Article
Determining the DNA sequence elements required for binding integration host factor to two different target sites.
Journal of bacteriology - 1 May 1994
Hales L M, Gumport R I, Gardner J F
Abstract excerpt
Binding sites for the Escherichia coli protein integration host factor (IHF) include a set of conserved bases that can be summarized by the consensus sequence WATCAANNNNTTR (W is dA or dT, R is dA or dG, and N is any nucleotide). However, additional 5'-proximal bases, whose common feature is a high dA+dT content, are also thought to be required for binding at some sites. We examine the relative contribution of...
Topics
- Bacterial Proteins
- Bacteriophage lambda
- Base Sequence
- Consensus Sequence
- DNA, Bacterial
- Escherichia coli
- Integration Host Factors
- Molecular Sequence Data
- Mutation
- Protein Binding
- Regulatory Sequences, Nucleic Acid