Article
Switching nucleotide specificity of Ha-Ras p21 by a single amino acid substitution at aspartate 119.
The Journal of biological chemistry - 28 Apr 1995
Zhong J M, Chen-Hwang M C, Hwang Y W
Abstract excerpt
We examined c-Ha-Ras harboring an aspartate to asparagine substitution at position 119 (mutation D119N). The Asp-119 is part of the conserved NKXD motif shared by members of the regulatory GTPase family. This asparagine residue has been proposed to participate in direct bonding to the guanine rin...
Topics
- Aspartic Acid
- Base Sequence
- GTP-Binding Proteins
- GTPase-Activating Proteins
- Hydrolysis
- Kinetics
- Molecular Sequence Data
- Mutation
- Nucleotides
- Oligodeoxyribonucleotides
- Protein Serine-Threonine Kinases
- Proteins
- Proto-Oncogene Proteins
- Proto-Oncogene Proteins c-raf
- Proto-Oncogene Proteins p21(ras)
- Substrate Specificity
- rap GTP-Binding Proteins
- ras GTPase-Activating Proteins