Article
Biochemical and biological consequences of changing the specificity of p21ras from guanosine to xanthosine nucleotides.
Oncogene - 4 Jan 1996
Schmidt G, Lenzen C, Simon I, Deuter R, Cool R H, Goody R S, Wittinghofer A
Abstract excerpt
The D119N mutation of p21ras was prepared by site-directed mutagenesis. Its nucleotide binding properties were investigated using fluorescently labelled guanosine and xanthosine nucleotides. Its affinity for guanosine nucleotides is severely reduced, with a concomitant increase in the affinity for xanthosine nucleotides, which leads to an almost complete reversal of base specificity. The protein is a GTPase as...
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