Article
Isolation and phenotypic characterization of lung fibroblasts.
Methods in molecular medicine - 1 Jan 2005
Baglole Carolyn J, Reddy Sireesha Y, Pollock Stephen J, Feldon Steven E, Sime Patricia J, Smith Terry J, Phipps Richard P
Abstract excerpt
Primary fibroblasts represent a heterogeneous population of cells that can be separated into subsets on the basis of cell surface markers such as Thy-1. Deriving fibroblasts initially involves obtaining tissue explants from tissues such as the lung, heart, cornea, skin, and orbit. The tissue is mechanically dissociated and cells are allowed to proliferate from the fragments. Following establishment of a primary...
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