Article
Novel use of a selectable fusion gene as an "in-out" marker for studying genetic loss in mammalian cells.
Molecular carcinogenesis - 1 Apr 1995
Trott D A, Cuthbert A P, Todd C M, Themis M, Newbold R F
Abstract excerpt
Recent demonstrations of loss of heterozygosity in a wide variety of human cancers suggest that large multilocus genetic deletions (presumably including tumor suppressor genes) constitute a major class of genetic alteration in human carcinogenesis. Here we show that a bifunctional fusion gene (Hytk), suitable for both positive and negative selection, is an effective marker for studying genetic loss in mammalian...
Topics
- 3T3 Cells
- 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide
- Animals
- Cell Line
- Cell Survival
- Chromosome Deletion
- Cloning, Molecular
- Dose-Response Relationship, Radiation
- Gene Deletion
- Genes, Tumor Suppressor