Functional inhibition of transitory proteins by intrabody-mediated retention in the endoplasmatic reticulum.

doi:10.1016/j.ymeth.2011.10.008

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Article

Functional inhibition of transitory proteins by intrabody-mediated retention in the endoplasmatic reticulum.

Methods (San Diego, Calif.) - 1 Mar 2012

Böldicke Thomas, Somplatzki Stefan, Sergeev Galina, Mueller Peter P

Abstract excerpt

Intrabodies are recombinantly expressed intracellular antibody fragments that can be used to specifically bind and inhibit the function of cellular proteins of interest. Intrabodies can be targeted to various cell compartments by attaching an appropriate localization peptide sequence to them. An efficient strategy with a high success rate is to anchor intrabodies in the endoplasmatic reticulum where they can...

Topics

Amino Acid Sequence
Animals
Antibodies
Base Sequence
Endoplasmic Reticulum
Gene Transfer Techniques
Genetic Vectors
Homologous Recombination
Humans
Molecular Sequence Data
Mutation
Neural Cell Adhesion Molecules
Peptide Library
Proteins
RNA, Antisense

Source

Open in PubMed

Identifiers

PMID: 22037249
DOI: 10.1016/j.ymeth.2011.10.008

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