Article
Intracellular expression of single chain antibodies reverts ErbB-2 transformation.
The Journal of biological chemistry - 30 Sept 1994
Beerli R R, Wels W, Hynes N E
Abstract excerpt
We report a novel approach for specific in vivo inactivation of the ErbB-2 receptor tyrosine kinase and suppression of ErbB-2-induced transformation. Genes encoding single chain antibodies that specifically bind to the extracellular domain of human ErbB-2 were constructed and expressed intracellu...
Topics
- 3T3 Cells
- Amino Acid Sequence
- Animals
- Cell Line, Transformed
- Endoplasmic Reticulum
- Humans
- Immunoglobulin Fragments
- Immunoglobulin Variable Region
- Mice
- Molecular Sequence Data
- Phenotype
- Receptor, ErbB-2
- Subcellular Fractions