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Article

Quantitation of tropoelastin mRNA and assessment of alternative splicing in human skin fibroblasts by reverse transcriptase-polymerase chain reaction.

PCR methods and applications - 1 Oct 1993

Holzenberger M, Levi-Minzi S A, Herzog C P, Deak S B, Robert L, Boyd C D

Abstract excerpt

We have developed a reverse transcriptase-polymerase chain reaction (RT-PCR) assay for the quantitative measurement of levels of tropoelastin mRNA in total RNA preparations from skin fibroblasts. This method facilitates the reproducible detection of low abundance tropoelastin mRNA in the range of 10-1000 copies per cell. The procedure is based on a competitive RT-PCR assay where a tropoelastin cDNA-derived...

Topics

Alternative Splicing
Base Sequence
Cells, Cultured
DNA Primers
Exons
Fibroblasts
Genotype
Humans
Molecular Sequence Data
Polymerase Chain Reaction
RNA, Messenger

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

RNA-Directed DNA Polymerase

Skin

Tropoelastin

Source

Identifiers

PMID: 7505678
DOI: 10.1101/gr.3.2.107

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7505678-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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