Article
The macroheterogeneity of recombinant human interferon-gamma produced by Chinese-hamster ovary cells is affected by the protein and lipid content of the culture medium.
Biotechnology and applied biochemistry - 1 Feb 1995
Castro P M, Ison A P, Hayter P M, Bull A T
Abstract excerpt
The culture environment exerts a major effect on the glycosylation pattern of recombinant human interferon-gamma (IFN-gamma) produced by Chinese-hamster ovary (CHO) cells. The recombinant IFN-gamma is heterogeneous and consists of a mixture of fully (2N), partially (1N) and non-glycosylated (0N) glycoforms, and throughout batch cultures there is a decline in the proportion of fully glycosylated IFN-gamma. Glucose...
Topics
- Animals
- CHO Cells
- Cell Line, Transformed
- Cells, Cultured
- Cricetinae
- Cricetulus
- Culture Media, Serum-Free
- Electrophoresis, Polyacrylamide Gel
- Glucose
- Glutamine
- Glycosylation