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Article

An empirical phase diagram approach to investigate conformational stability of "second-generation" functional mutants of acidic fibroblast growth factor-1.

Protein science : a publication of the Protein Society - 1 Mar 2012

Alsenaidy Mohammad A, Wang Tingting, Kim Jae Hyun, Joshi Sangeeta B, Lee Jihun, Blaber Michael, Volkin David B, Middaugh C Russell

Abstract excerpt

Acidic fibroblast growth factor-1 (FGF-1) is an angiogenic protein which requires binding to a polyanion such as heparin for its mitogenic activity and physicochemical stability. To evaluate the extent to which this heparin dependence on solution stability could be reduced or eliminated, the structural integrity and conformational stability of 10 selected FGF-1 mutants were examined as a function of solution pH...

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Identifiers

PMID: 22113934
DOI: 10.1002/pro.2008
PMCID: PMC3375442

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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