Article
Polymerase chain reaction detection of a novel human KIT (mast/stem cell growth factor receptor) gene polymorphism by single-strand conformation polymorphism analysis or by SmaI or BstNI cleavage.
Human genetics - 1 Sept 1993
Spritz R A, Holmes S A
Abstract excerpt
We describe a common single-base polymorphism of the KIT gene that alters both SmaI and BstNI restriction sites, but is most easily detected as a single-strand conformation polymorphism (SSCP) using the polymerase chain reaction (PCR).
Topics
- Alleles
- Base Sequence
- Chromosomes, Human, Pair 4
- DNA, Single-Stranded
- Deoxyribonucleases, Type II Site-Specific
- Gene Frequency
- Hematopoietic Cell Growth Factors
- Humans
- Molecular Sequence Data
- Nucleic Acid Conformation
- Piebaldism