Article
Isolation of a macrophage-like cell line defective in binding of lipopolysaccharide. Influence of serum and lipopolysaccharide chain length on macrophage activation.
Journal of immunology (Baltimore, Md. : 1950) - 1 Sept 1993
Kirikae T, Schade F U, Kirikae F, Rietschel E T, Morrison D C
Abstract excerpt
A mutant cell line (J7.DEF.3) derived from murine macrophage-like J774.1 cells, was isolated on the basis of defective specific 125I-labeled LPS-binding in the presence of serum. Although J7.DEF.3 cells still respond to LPS in inducing TNF-alpha release and nitric oxide (NO) formation, these cell...
Topics
- Animals
- Antigens, CD
- Antigens, Differentiation, Myelomonocytic
- Blood Physiological Phenomena
- Cell Line
- Lipid A
- Lipopolysaccharide Receptors
- Lipopolysaccharides
- Macrophage Activation
- Macrophages
- Mice
- Mice, Inbred BALB C
- Mutation
- Nitric Oxide
- Structure-Activity Relationship
- Tumor Necrosis Factor-alpha