Maintenance of hematopoiesis in serum-free bone marrow cultures involves sequential recruitment of quiescent progenitors.

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Article

Maintenance of hematopoiesis in serum-free bone marrow cultures involves sequential recruitment of quiescent progenitors.

Experimental hematology - 1 Sept 1993

Lansdorp P M, Dragowska W

Abstract excerpt

We previously described that cells with a CD34+CD71lo phenotype from adult human bone marrow are maintained at constant numbers in long-term suspension cultures supplemented with interleukin-6 (IL-6), IL-3, mast growth factor (MGF) (a c-kit ligand), and erythropoietin (Epo). In view of the large increase in cell numbers in such cultures (for example, > 10(6)-fold per cell), this was an unexpected finding. The...

Topics

Antigens, CD
Antigens, CD34
Antigens, Differentiation, B-Lymphocyte
Blood
Bone Marrow Cells
Cell Division
Cell Survival
Cells, Cultured
Clone Cells
Erythropoietin
Flow Cytometry

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Hematopoiesis

Hematopoietic Cell Growth Factors

Hematopoietic Stem Cells

Humans

Interleukin-3

Interleukin-6

Kinetics

Source

Open in PubMed

Identifiers

PMID: 7689481

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7689481-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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