Article
Intrinsic potential of phenotypically defined human hemopoietic stem cells to self-renew in short-term in vitro cultures.
Experimental hematology - 1 Sept 1999
Knaän-Shanzer S, Verlinden S F, van Beusechem V W, Van Bekkum D W, Valerio D
Abstract excerpt
In search for culture conditions that will facilitate hemopoietic stem cell (HSC) replication while preserving their primitive properties, we have made use of a multi-parameter FACS assay to define HSCs on basis of their phenotypic characteristics, i.e., CD34++CD33,38,71(-). Bone marrow and umbilical cord blood samples of CD34(+) cells from 31 donors were loaded with the membrane dye PKH26 and each exposed to...
Topics
- Adult
- Animals
- Antigens, CD
- Blood Cells
- Bone Marrow Cells
- Cattle
- Cell Culture Techniques
- Cell Division
- Cells, Cultured
- Coculture Techniques
- Colony-Forming Units Assay
- Culture Media
- Culture Media, Serum-Free
- Fetal Blood