Article
Long-term erythropoiesis from constant numbers of CD34+ cells in serum-free cultures initiated with highly purified progenitor cells from human bone marrow.
The Journal of experimental medicine - 1 Jun 1992
Lansdorp P M, Dragowska W
Abstract excerpt
To directly study the biological properties of purified hematopoietic colony-forming cell precursors, cells with a CD34+ CD45RAlo CD71lo phenotype were purified from human bone marrow using density separation and fluorescence-activated cell sorting, and were cultured in serum-free culture medium...
Topics
- Antigens, CD
- Antigens, CD34
- Bone Marrow Cells
- Cell Separation
- Cell Survival
- Cells, Cultured
- Centrifugation, Density Gradient
- Colony-Forming Units Assay
- Culture Media, Serum-Free
- Cytokines
- DNA Replication
- Erythropoiesis
- Erythropoietin
- Flow Cytometry
- Hematopoietic Cell Growth Factors
- Hematopoietic Stem Cells
- Humans
- Interleukin-3