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Article

Long-term erythropoiesis from constant numbers of CD34+ cells in serum-free cultures initiated with highly purified progenitor cells from human bone marrow.

The Journal of experimental medicine - 1 Jun 1992

Lansdorp P M, Dragowska W

Abstract excerpt

To directly study the biological properties of purified hematopoietic colony-forming cell precursors, cells with a CD34+ CD45RAlo CD71lo phenotype were purified from human bone marrow using density separation and fluorescence-activated cell sorting, and were cultured in serum-free culture medium supplemented with various cytokines. In the presence of interleukin 3 (IL-3), IL-6, erythropoietin, and mast cell...

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Identifiers

PMID: 1375263
DOI: 10.1084/jem.175.6.1501
PMCID: PMC2119250

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