Article
Characterization of gap junctional communication-deficient mutants of a rat liver epithelial cell line.
European journal of cell biology - 1 Apr 1993
Oh S Y, Dupont E, Madhukar B V, Briand J P, Chang C C, Beyer E, Trosko J E
Abstract excerpt
To understand the mechanism(s) regulating gap junctional communication, we isolated gap junctional intercellular communication-deficient (GJIC-) mutant clones of a rat liver epithelial cell line, WB F-344, which is hypoxanthine-guanine phosphoribosyl transferase deficient (HGPRT-). The cells were exposed to a mutagenesis regimen and cocultured with the wild type HGPRT+ cells. Four GJIC- and one positive clones...
Topics
- Animals
- Blotting, Western
- Cell Communication
- Cell Line
- Connexins
- Epithelium
- Hypoxanthine Phosphoribosyltransferase
- Intercellular Junctions
- Isoquinolines
- Liver
- Membrane Proteins