Article
Restoration of gap-junctional intercellular communication in a communication-deficient rat liver cell mutant by transfection with connexin 43 cDNA.
Molecular carcinogenesis - 1 Jan 1993
Jou Y S, Matesic D, Dupont E, Lu S C, Rupp H L, Madhukar B V, Oh S Y, Trosko J E, Chang C C
Abstract excerpt
To study the biochemical basis of gap-junctional intercellular communication (GJIC) and its role in tumorigenesis, a mammalian cell expression vector carrying both a rat connexin 43 (Cx43) cDNA and an amplifiable dihydrofolate reductase (DHFR) gene was transfected into the GJIC-deficient rat live...
Topics
- Animals
- Blotting, Northern
- Blotting, Southern
- Blotting, Western
- Cell Communication
- Connexin 43
- DNA, Complementary
- Gap Junctions
- Gene Amplification
- Gene Expression
- Liver
- Liver Neoplasms, Experimental
- Methotrexate
- Mutation