Article
Analysis of electroporation-induced genetic damages in V79/AP4 Chinese hamster cells.
Mutation research - 1 Jun 1993
Vatteroni L, Piras A, Simi S, Mariani L, Moretti A, Citti L, Mariani T, Rainaldi G
Abstract excerpt
Electroporation is a recent technique used to introduce exogenous DNA into eukaryotic cells. It is important to establish that the gene of interest is transferred into a functional, non-mutated recipient cell. V79/AP4 Chinese hamster cells were exposed to high-voltage pulsed electric fields and some biological and genetic effects were measured. The results showed that cytotoxicity was related in a dose-dependent...
Topics
- Animals
- Cell Survival
- Cells, Cultured
- Chromosome Aberrations
- Cricetinae
- Cricetulus
- DNA Damage
- Electricity
- Hypoxanthine Phosphoribosyltransferase
- Mutation
- Polymerase Chain Reaction