Article
The secondary structure and sequence optimization of an RNA ligase ribozyme.
Nucleic acids research - 25 Aug 1995
Ekland E H, Bartel D P
Abstract excerpt
In vitro selection can generate functional sequence variants of an RNA structural motif that are useful for comparative analysis. The technique is particularly valuable in cases where natural variation is unavailable or non-existent. We report the extension of this approach to a new extreme--the...
Topics
- Base Sequence
- Cloning, Molecular
- DNA Primers
- Genetic Variation
- Kinetics
- Molecular Sequence Data
- Nucleic Acid Conformation
- RNA Ligase (ATP)
- RNA, Catalytic
- Sequence Homology, Nucleic Acid
- Substrate Specificity