Article
Design of hairpin ribozyme variants with improved activity for poorly processed substrates.
The FEBS journal - 1 Feb 2011
Drude Irene, Strahl Anne, Galla Daniel, Müller Oliver, Müller Sabine
Abstract excerpt
Application of ribozymes for knockdown of RNA targets requires the identification of suitable target sites according to the consensus sequence. For the hairpin ribozyme, this was originally defined as Y⁻² N⁻¹ *G+¹ U+² Y+³ B+⁴, with Y = U or C, and B = U, C or G, and C being the preferred nucleobase at positions -2 and +4. In the context of development of ribozymes for destruction of an oncogenic mRNA, we have...
Topics
- Base Sequence
- Hydrogen Bonding
- Kinetics
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- RNA, Catalytic
- RNA, Messenger
- Substrate Specificity
- beta Catenin