Isolation of a ribozyme with 5'-5' ligase activity.

doi:10.1016/1074-5521(95)90051-9

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Article

Isolation of a ribozyme with 5'-5' ligase activity.

Chemistry & biology - 1 May 1995

Chapman K B, Szostak J W

Abstract excerpt

BACKGROUND: Many new ribozymes, including sequence-specific nucleases, ligases and kinases, have been isolated by in vitro selection from large pools of random-sequence RNAs. We are attempting to use in vitro selection to isolate new ribozymes that have, or can be evolved to have, RNA polymerase-...

Topics

Base Sequence
Chromatography, Thin Layer
Cloning, Molecular
DNA-Directed RNA Polymerases
Directed Molecular Evolution
Ligases
Molecular Sequence Data
Mutation
Oligonucleotides
RNA, Catalytic
Single-Strand Specific DNA and RNA Endonucleases

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