Protein phosphatase inhibitors, okadaic acid and calyculin A, induce alkaline phosphatase activity in osteoblastic cells derived from newborn mouse calvaria.

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Back to search

Article

Protein phosphatase inhibitors, okadaic acid and calyculin A, induce alkaline phosphatase activity in osteoblastic cells derived from newborn mouse calvaria.

Biochemistry and molecular biology international - 1 Jun 1995

Murata T, Shirakawa S, Takehara T, Kobayashi S, Haneji T

Abstract excerpt

To determine whether protein phosphatases can affect bone regulation, we examined the effects of okadaic acid (OA) and calyculin A (CA), specific inhibitors of protein phosphatases type 1 and type 2A, on alkaline phosphatase activity of mouse osteoblastic cells. Clone MC3T3-E1 cells were cultured with varying concentrations of OA and CA. OA and CA stimulated alkaline phosphatase (ALP) activity in the cells in...

Topics

Alkaline Phosphatase
Amino Acids
Animals
Animals, Newborn
Clone Cells
Enzyme Induction
Enzyme Stability
Ethers, Cyclic
Marine Toxins
Mice
Okadaic Acid

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Osteoblasts

Oxazoles

Phenotype

Phosphoprotein Phosphatases

Phosphorylation

Skull

Temperature

Source

Open in PubMed

Identifiers

PMID: 7663440

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7663440-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 11 closest

Protein phosphatase inhibitors, okadaic acid and calyculin A, induce alkaline phosphatase activity in osteoblastic cells derived from newborn mouse calvaria.PMID 7663440Read ↗

Osteoblast tissue-nonspecific alkaline phosphatase antagonizes and regulates PC-1.Semantic proximity Read ↗

Optimisation of the differing conditions required for bone formation in vitro by primary osteoblasts from mice and ratsSemantic proximity Read ↗

Cellular and molecular phenotypes of osteogenic cells isolated from the medullary bone of the hen in vitro.Semantic proximity Read ↗

Inorganic polyphosphate induces osteoblastic differentiation.Semantic proximity Read ↗

Matrix mineralization and the differentiation of osteocyte-like cells in culture.Semantic proximity Read ↗

Calyculin A induces apoptosis and stimulates phosphorylation of p65NF-kappaB in human osteoblastic osteosarcoma MG63 cells.Semantic proximity Read ↗

Establishment of an osteoblast-like cell line, MMC2, from p53-deficient mice.Semantic proximity Read ↗

Okadaic acid activates the PKR pathway and induces apoptosis through PKR stimulation in MG63 osteoblast-like cells.Semantic proximity Read ↗

Effects of bisphosphonates on alkaline phosphatase activity, mineralization, and prostaglandin E2 synthesis in the clonal osteoblast-like cell line MC3T3-E1Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.