Article
[Study on the simultaneous genotyping of human platelet antigens of 1, 2, 3, 4, 5, 6 system by PCR-SSP and its applications].
Yi chuan = Hereditas - 1 Sept 2004
Deng Zhi-Hui, Wu Guo-Guang, Li Da-Cheng
Abstract excerpt
To set up the simultaneous genotyping of human platelet antigens of 1,2,3,4,5,6 system by PCR-SSP assay and use the genotyping method for the study of platelet antigens. In this study, 18 sequence-specific primers were designed and synthesized. The annealing temperature for all sequence-specific primer pair, the concentration of each primer pair and the concentration of Mg2+ were adjusted to the optimum so that...
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