Rapid determination of platelet alloantigen genotypes by polymerase chain reaction using allele-specific primers.

doi:10.1046/j.1537-2995.1994.341195065032.x

Back to search

Article

Rapid determination of platelet alloantigen genotypes by polymerase chain reaction using allele-specific primers.

Transfusion - 1 Jan 2000

Skogen B, Bellissimo D B, Hessner M J, Santoso S, Aster R H, Newman P J, McFarland J G

Abstract excerpt

BACKGROUND: The technique of allele-specific polymerase chain reaction (PCR) amplification has been adapted for DNA-based human platelet alloantigen typing. STUDY DESIGN AND METHODS: Sequence-specific primers were used to discriminate between the alleles encoding the six major human platelet allo...

Topics

Alleles
Antigens, Human Platelet
Epitopes
Genotype
Heterozygote
Homozygote
Humans
Molecular Sequence Data
Polymerase Chain Reaction

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.