Article
Rapid determination of platelet alloantigen genotypes by polymerase chain reaction using allele-specific primers.
Transfusion - 1 Jan 2000
Skogen B, Bellissimo D B, Hessner M J, Santoso S, Aster R H, Newman P J, McFarland J G
Abstract excerpt
BACKGROUND: The technique of allele-specific polymerase chain reaction (PCR) amplification has been adapted for DNA-based human platelet alloantigen typing. STUDY DESIGN AND METHODS: Sequence-specific primers were used to discriminate between the alleles encoding the six major human platelet alloantigens in a series of patients and normal blood donors. RESULTS: This technique allows the direct determination of...
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