Article
Myristoyl modification of viral proteins: assays to assess functional roles.
Methods in enzymology - 1 Jan 1995
Chow M, Moscufo N
Abstract excerpt
Characterization of the effects of myristoylation on poliovirus function has largely depended on the availability of methods previously characterized to study various aspects of virus biology. Those methods are described here to provide specific examples of how they may be used to analyze the myristoylation mutants and to illustrate general approaches. It is possible that the poliovirus protocols may be directly...
Topics
- Capsid
- Cell Membrane
- Crystallography, X-Ray
- Dactinomycin
- Electrophoresis, Polyacrylamide Gel
- Endopeptidases
- HeLa Cells
- Humans
- Indicators and Reagents
- Kinetics
- Methionine
- Mutation
