Article
A Gly1 to Ala substitution in poliovirus capsid protein VP0 blocks its myristoylation and prevents viral assembly.
The Journal of general virology - 1 May 1991
Marc D, Girard M, van der Werf S
Abstract excerpt
Capsid protein VP4 of poliovirus is acylated with myristic acid via an amide linkage to its N-terminal glycine residue. Our previous studies suggested that myristic acid plays a role in poliovirus assembly and in the early events of infection. In order to understand better its role in the assembly process, we introduced a Gly1 to Ala amino acid substitution in the myristoylation signal sequence of VP4. This...
Topics
- Alanine
- Amino Acid Sequence
- Base Sequence
- Capsid
- Capsid Proteins
- Electrophoresis, Polyacrylamide Gel
- Glycine
- HeLa Cells
- Humans
- Molecular Sequence Data
- Mutation